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目的在重组人骨形态发生蛋白-2(rhBMP-2)诱导异位成骨过程中,局部应用不同浓度的辛伐他汀(SIM),探讨SIM对rhBMP-2诱导成骨的作用。方法①以牛松质骨为原料经过脱脂、脱蛋白等步骤制备牛骨基质明胶载体,将辛伐他汀和rhBMP-2分别与BMG载体复合;②将45只雄性SD大鼠随机分为3组。A组:高剂量实验组;B组:低剂量实验组;C组:对照组;③将复合体植入大鼠股后部肌袋中,术后15d、30d、60d对实验大鼠进行大体观察、X线检测。结果①大体观察:各组组织块均随着时间延长而变硬,范围变大;②X线结果,各组植入部位术后15d、30d未见显影,于术后60d各组可见不同密度的影像,B组和C组钙化明显,成高密度影像;A组成低密度影像。成骨区灰度值A组明显低于B组和C组。结论高浓度辛伐他汀可明显抑制rhBMP-2的促进成骨作用;低浓度辛伐他汀的抑制作用则不明显;证实了rhBMP-2在促进成骨方面有重要作用。
Objective To investigate the effect of simvastatin on osteogenic differentiation induced by rhBMP-2 induced by recombinant human bone morphogenetic protein-2 (rhBMP-2) in different levels of simvastatin (SIM). Methods ① Bovine bone matrix gelatin carrier was prepared by using bovine cancellous bone as raw material, degreasing and deproteinization, simvastatin and rhBMP-2 were respectively combined with BMG carrier; ② 45 male SD rats were randomly divided into 3 groups . Group A: high-dose experimental group; Group B: low-dose experimental group; Group C: control group; ③ The composite was implanted into the posterior femoral muscle bags of rats and the rats were sacrificed on the 15th, Observation, X-ray examination. Results ① Gross observation: The histological sections in each group were hardened with the extension of time, and the range became larger. ②X-ray results showed that there was no visualization at 15d and 30d after implantation, Image, B group and C group calcification obvious, into a high-density images; A composition of low-density images. The gray value of osteoblast in group A was significantly lower than that in group B and C. Conclusions High concentration of simvastatin can significantly inhibit the osteogenic effect of rhBMP-2. The inhibitory effect of simvastatin at low concentration is not obvious. It is confirmed that rhBMP-2 plays an important role in promoting osteogenesis.