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目的对中国部分省份屠宰场鸡胴体分离的空肠弯曲菌进行分子分型和溯源分析。方法采用荧光标记扩增片段长度多态性技术(FAFLP),对72株空肠弯曲菌分离株进行分子分型,选用HindⅢ和HhaⅠ限制性内切酶进行双酶切,电泳结果经Gene Marker V1.80处理后,导入Bio Numerics软件进行聚类分析,所得结果收入溯源分析数据库。结果 72株空肠弯曲菌分离株共产生241条多态性片段,多数在80~100条;72株菌共产生72种带型,分辨率为100%;以70%相似度为界,可以分为11个群,群间最低相似度为56.9%,群内最高相似度为94.9%。以80%相似度为界,可将A群分为5个亚群,B群分为15个亚群,多个亚群所包含菌株显示有完全地域同源性。结论 FAFLP方法分辨率高,聚类分析显示有一定同源性,适用于空肠弯曲菌的分子分型和溯源分析。
Objective To analyze the molecular typing and traceability of Campylobacter jejuni isolated from chicken carcasses in slaughterhouses in some provinces in China. Methods 72 strains of Campylobacter jejuni isolates were genotyped by fluorescence-labeled amplified fragment length polymorphism (FAFLP), and double digested with restriction endonuclease Hind Ⅲ and Hha Ⅰ. The results of electrophoresis were analyzed by Gene Marker V1. After 80 treatment, the software was imported into Bio Numerics for cluster analysis, and the results were collected into the traceability analysis database. Results 72 strains of Campylobacter jejuni isolated a total of 241 polymorphic fragments, mostly in 80 to 100; 72 strains produced a total of 72 bands with a resolution of 100%; with 70% similarity as a boundary, can be divided The lowest similarity among groups was 56.9%, and the highest similarity among groups was 94.9%. With 80% similarity as a boundary, A group can be divided into five subgroups, B group is divided into 15 subgroups, multiple subgroups contained strains showed complete geographical homology. Conclusion FAFLP method has high resolution and clustering analysis shows some homology, which is suitable for molecular typing and traceability analysis of Campylobacter jejuni.