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目的构建两种博尔纳病病毒株持续感染的PC-12细胞模型,为研究博尔纳病病毒感染致病机制及比较两种病毒株致病特点的差别提供工具。方法将BDV Strain V株和Hu株分别感染PC-12细胞系,并进行传代培养,最后通过Real time FQ RT-PCR、Western blot、间接免疫荧光方法进行病毒核酸和蛋白的检测。结果在培养传代6代后,两种病毒株感染的PC-12细胞均可检测到BDV核酸及蛋白。结论 BDV Strain V株和Hu株均可在PC-12细胞中复制和表达,两种博尔纳病病毒株持续感染PC-12细胞模型构建成功。
OBJECTIVE: To construct a PC-12 cell model of persistent infection with Borna disease virus strains and to provide a tool for studying the pathogenesis of Borna disease virus infection and comparing the differences in pathogenicity between the two strains. Methods PCV-12 cell lines were infected with Strain V strain and Hu strain respectively. The virus DNA and protein were detected by Real time FQ RT-PCR, Western blot and indirect immunofluorescence. Results After cultured for 6 passages, both BDV nucleic acid and protein were detected in PC-12 cells infected by both strains. Conclusion Both strains of Strain V and Hu of BDV can be replicated and expressed in PC-12 cells. The two strains of PCV-12 were successfully infected in PC-12 cells.