本实验引用一对金钱白花蛇的特异性引物BuL-1和BuH-1,用聚合酶链反应方法(以下简称PCR)对金钱白花蛇、大白花锦蛇、赤链游蛇和蕲蛇等五种蛇样品同时进行扩增、琼脂糖凝胶电泳,并对电泳结果进行分析。结果显示,金钱白花蛇样品扩增产物可见一条230bp的目的片段,而其它四种蛇样品扩增后未见扩增产物出现。本法简单、快速、特异性强、微量。 In this experiment, a pair of specific primers BuL-1 and BuH-1 were used, and a polymerase chain reaction (PCR) method was used for the analysis of Pleurotus citrinum, Blet. Snake samples were simultaneously amplified, agarose gel electrophoresed, and electrophoresis results were analyzed. The results showed that there was a 230 bp target fragment in the amplified product of P. viridis, but no amplification product was observed after amplification of the other four snakes. This method is simple, rapid, specific, and trace.