论文部分内容阅读
用DNA 3′ 末端标记、免疫组化和原位杂交方法 ,通过连续切片比较研究了相同卵泡颗粒细胞抑制素亚基和LH受体 (LHR)与卵细胞tPA表达和卵泡闭锁的关系 .实验结果表明 :( 1 )卵泡闭锁伴随卵细胞tPA活性明显增加 ;( 2 )颗粒细胞抑制素的产生调节卵细胞tPA活性的表达并与卵泡发育状态密切相关 ;( 3)卵泡闭锁时 ,颗粒细胞几乎不表达LHR和抑制素亚基 .上述结果提示 :卵细胞的tPA在闭锁卵泡中可能参与卵细胞的自我瓦解和清除过程 ;颗粒细胞表达的抑制素可能是tPAmRNA翻译的一种抑制因子 ,如其表达受阻 ,可导致卵细胞tPA蛋白活性增加引起卵泡闭锁 .
The relationship between granulocystostatin subunit and LH receptor (LHR) of the same follicle and tPA expression and follicular atresia in the same follicle was studied by DNA 3 ’end labeling, immunohistochemistry and in situ hybridization. The experimental results show that (1) follicular atresia accompanied by significantly increased tPA activity in ovary; (2) the production of granulosin regulates the expression of tPA in ovary and closely related to follicular development; (3) When follicular atresia, granulocytes hardly express LHR and Inhibin subunits.The above results suggest that the tPA of egg cells may be involved in the process of egg cell self-disassembly and clearance in atresia follicles. The inhibin expressed by granulosa cells may be an inhibitor of tPA mRNA translation. Increased protein activity causes follicles atresia.