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目的观察分析静脉注射氯胺酮家兔死后血液流动对体内药物浓度再分布影响。方法雄性新西兰大白兔随机分为实验组2组(各24只),对照组(8只);实验组家兔经耳缘静脉注入40mg/kg氯胺酮,1.5h后处死,其中一组立即结扎主动脉,另一组不结扎;家兔尸体仰卧位室温下保存,分别于死后0、3、6、12、24、48、72和96h解剖并采取组织和体液样本;对照组静脉注射等量生理盐水,同样方法解剖取相同样本。所有样本采用GC/MS和GC-NPD法检测样品中氯胺酮含量。结果两个实验组家兔尸体放置96h内氯胺酮含量,除尿液各时间点与0h以及相邻时间点之间比较均有显著性差异(P<0.05)外,两组家兔其余各类样本与0h以及相邻时间点比较均无显著性差异(P>0.05)。除尿液外,各类样本两个实验组之间均无显著性差异(P>0.05);结扎组和不结扎组心血与外周血中氯胺酮含量比值分别为0.90~1.03和0.90~1.02。结论静脉注射氯胺酮家兔死后的血液流动不是体内氯胺酮发生再分布的主要机制。
Objective To observe and analyze the influence of blood flow in rabbits after intravenous injection of ketamine on the redistribution of drug in vivo. Methods Male New Zealand white rabbits were randomly divided into experimental group (n = 24) and control group (n = 8). The rabbits in the experimental group were injected with 40 mg / kg ketamine via the ear vein and sacrificed 1.5 h later. Artery, and the other group was not ligated. The carcass of the rabbits was stored in supine position at room temperature, and the samples of tissues and body fluids were dissected at 0, 3, 6, 12, 24, 48, 72 and 96 h after death. Physiological saline, the same method to dissect the same sample. All samples were tested for ketamine content by GC / MS and GC-NPD methods. Results The contents of ketamine in the 96-h rabbits in both experimental groups were significantly lower than those in the control group (P0.01) There was no significant difference between 0h and adjacent time points (P> 0.05). Except for urine, there was no significant difference between the two experimental groups (P> 0.05). The ratio of ketamine between the two groups was 0.90 ~ 1.03 and 0.90 ~ 1.02 respectively in the ligation and non-ligating groups. Conclusion The blood flow of rabbits after intravenous injection of ketamine is not the main mechanism of the redistribution of ketamine in vivo.