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本文采用将尿样经酸水解后,用有机溶剂提取一次,以自制硅胶G薄层板层析检查。一、方法取检验者尿样,在盐酸酸性条件下,沸水浴水解,游离出吗啡。用氢氧化钠试液中和至pH 9.0,加入一定量pH 9.3的碳酸钠-碳酸氢钠缓冲液及一定量氯仿:乙醇(9∶1)提取液。振摇后,静置分离。提取液在水浴上蒸发至干,将残渣用适量提取液溶解,点于硅胶G薄层板上,并且同一块薄层板上依次点一定量吗啡标准液及同样品液方法处理的加入10 mg吗啡标准液
In this paper, the urine samples were acid-hydrolyzed, extracted once with organic solvents, and self-made silica gel G TLC. First, the method to take the test urine samples, acidic conditions in hydrochloric acid, boiling water bath hydrolysis, free morphine. Neutralization with sodium hydroxide test solution to pH 9.0, add a certain amount of pH 9.3 sodium bicarbonate - sodium bicarbonate buffer and a certain amount of chloroform: ethanol (9: 1) extract. After shaking, stand for separation. The extract was evaporated to dryness on a water bath, the residue was dissolved with an appropriate amount of extract, point in silica gel G plate, and the same piece of thin plate in order to point a certain amount of morphine standard solution and the same liquid method of treatment by adding 10 mg Morphine standard solution