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目的观察低剂量毒死蜱和氯氰菊酯联合染毒对仔代雄性大鼠生殖功能的影响及其机理。方法健康Wistar大鼠雌雄按2∶1合笼,孕鼠分为对照组、毒死蜱组、氯氰菊酯组、毒死蜱和氯氰菊酯联合染毒组等4组,每组8只,孕l至孕8天染毒孕鼠。给予雄性仔鼠普通饮食喂养至成年。股动脉放血法处死仔鼠,迅速分离脏器,计算脏器系数,附睾尾精子计数,计算精子活动率、精子畸形率。测定血清生殖激素和乙酰胆碱酯酶活力,观察睾丸组织病理改变、生殖细胞凋亡和生精细胞端粒酶的表达。结果联合染毒组雄性仔鼠睾丸、附睾的体重比值显著性降低(P<0.05),附睾尾精子总数显著低于对照组,血清FSH高于对照组,T低于对照组(P<0.05)。联合染毒组雄性仔鼠睾丸少量曲细精管生精上皮退化变性,生精细胞脱落,生精细胞凋亡指数明显高于对照组(P<0.05)。结论胚胎期毒死蜱和氯氰菊酯混合染毒对仔代大鼠的生殖系统有明显的增毒作用;对睾丸的损伤及性激素水平(FSH、T)的协同影响,可能是产生联合生殖毒性的机理之一;提示对毒死蜱和氯氰菊酯进行环境健康风险评价时应考虑这种协同作用。
Objective To observe the effect and mechanism of combined treatment of low dose chlorpyrifos and cypermethrin on reproductive function of offspring male rats. Methods Healthy Wistar rats were male and female with 2: 1 cages. Pregnant rats were divided into 4 groups: control group, chlorpyrifos group, cypermethrin group, chlorpyrifos and cypermethrin combined group. Pregnant rats. Male offspring were given normal diet to adulthood. Femoral artery bleeding method of sacrificing pups, rapid separation of organs, organ coefficient calculation, epididymal sperm count, calculation of sperm motility, sperm deformity rate. Determination of serum reproductive hormones and acetylcholinesterase activity, testicular histopathology, germ cell apoptosis and telomerase expression of spermatogenic cells. Results The body weight ratio of testis and epididymis were significantly decreased (P <0.05), the total number of cauda equina epididymal sperm was significantly lower than that of control group, serum FSH was higher than that of control group, T was lower than that of control group (P <0.05) . In the joint exposure group, a small amount of testicular seminiferous tubules degeneration and degeneration of spermatogenic cells, spermatogenic cells shedding, spermatogenic cell apoptosis index was significantly higher than that of the control group (P <0.05). Conclusion The combination of chlorpyrifos and cypermethrin during embryogenesis has obvious toxic effects on the reproductive system of offspring rats. The synergistic effect of testicular injury and sex hormones (FSH, T) may be one of the mechanisms of joint reproductive toxicity ; Suggesting that this synergistic effect should be considered when assessing environmental health risks of chlorpyrifos and cypermethrin.