目的:在虫草甘露醇含量测定过程中,常用的两种预处理方法是水提取法和醇提取法。为提高测定结果的准确性,对此进行多重分析比较。方法:以经典的氧化还原滴定法为基础,对水提取法和醇提取法进行直接比较,交叉比较,综合比较,相对误差分析。结果:水提取法的测出量达到虫草甘露醇实际含量的95%以上,且相对误差很小;醇提取法的测出量则不足其实际含量的70%,且相对误差较大。结论:在虫草甘露醇含量的测定中,样品预处理采用水提取法的准确性和精确性均大大高于醇提取法。 Objective: In the determination of Cordyceps mannitol content, two commonly used pretreatment methods are water extraction and alcohol extraction. In order to improve the accuracy of the measurement results, multiple analysis and comparison were performed. Methods: Based on the classic redox titration method, direct comparison of water extraction and alcohol extraction, cross comparison, comprehensive comparison and relative error analysis were conducted. Results: The measured amount of water extraction method was more than 95% of the actual content of Cordyceps militaris, and the relative error was small; the amount of alcohol extraction was less than 70% of the actual content, and the relative error was large. Conclusion: In the determination of mannitol content in Cordyceps militaris, the accuracy and accuracy of water pretreatment for sample pretreatment are significantly higher than those for alcohol extraction.