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本文对干旱胁迫下转酵母TPS1基因玉米的根系生长进行了研究。结果显示,经干旱胁迫后,转基因玉米的根系较野生型相比,根长和直径分别增加了79.3%和13.3%,一级、二级侧根数、根系的总吸收面积、活跃吸收面积、比表面积和根系活力较野生型也极显著增加。对根系组织观察发现,转TPS1基因玉米植株的根系细胞较大,细胞纵向和横向显著增长。转基因玉米根系的生长素含量较野生型的高,而其细胞分裂素含量则低。进一步对玉米根系生长相关基因的表达水平分析发现,与生长素有关的根系生长正调控基因PLT1、PIN1、AUX/IAA、CRL1表达较野生型上调,其中PLT1、AUX/IAA、CRL1的表达量比野生型的高2倍以上,而与细胞分裂素有关的根系生长负调控基因WOX11、ARR2、ARR1表达较野生型下调,比野生型的下降50%以上。
The root growth of TPS1 transgenic maize under drought stress was studied in this paper. The results showed that after drought stress, the root length and diameter of transgenic corn increased by 79.3% and 13.3% respectively compared with wild type. The root and secondary root number, the total absorption area and active absorption area of root system, The surface area and root activity also increased significantly more than the wild type. Root tissue observation found that transgenic TPS1 transgenic corn plants had larger root cells and significantly longer cells in both vertical and horizontal directions. The auxin content of transgenic corn roots was higher than that of wild type, while the content of cytokinin was low. Further analysis of the expression levels of genes related to root growth in maize revealed that the expression of PLT1, PIN1, AUX / IAA and CRL1 were upregulated compared with wild type, and the expression levels of PLT1, AUX / IAA and CRL1 Compared with the wild-type, the expression of WOX11, ARR2 and ARR1 was down-regulated by more than 50% compared with the wild-type.