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在强碱性介质中,CdTe量子点(QDs)对鲁米诺-KIO4化学发光体系具有强烈的增敏发光作用,而在此发光体系中加入对乙酰氨基酚(APAP)又会产生抑制发光现象。基于对乙酰氨基酚(APAP)对CdTe QDs-鲁米诺-KIO4碱性化学发光体系的定量发光猝灭现象,建立了测定药物制剂中对乙酰氨基酚含量的流动注射-化学发光分析新方法。考察了CdTe QDs浓度、鲁米诺溶液浓度、KIO4溶液浓度、NaOH溶液浓度和泵流速等对测试结果的影响。在优化的最佳测定条件下,化学发光(CL)强度猝灭值的对数值与对乙酰氨基酚质量浓度的对数值之间有良好的线性关系,线性范围为1.0×10-7~1.0×10-5g/mL,检出限6.0×10-8g/mL(3σ),测定的相对标准偏差为1.3%(n=10)。方法已用于对乙酰氨基酚片剂中对乙酰氨基酚含量的检测,加标回收率在94.5%~105.0%之间。
In strong alkaline medium, CdTe quantum dots (QDs) have a strong sensitizing effect on luminol-KIO4 chemiluminescence system, while the addition of acetaminophen (APAP) in this luminescent system can inhibit the luminescence . Based on the quantitative luminescence quenching of acetaminophen (APAP) to CdTe QDs-luminol-KIO4 basic chemiluminescence system, a new flow-injection chemiluminescence (PLGA) method for the determination of acetaminophen in pharmaceutical preparations was established. The effects of CdTe QDs concentration, luminol solution concentration, KIO4 solution concentration, NaOH solution concentration and pump flow rate on the test results were investigated. Under the optimized conditions, there was a good linear relationship between the logarithm of quenching value of chemiluminescence (CL) and the logarithm of mass concentration of acetaminophen, the linear range was 1.0 × 10-7 ~ 1.0 × The detection limit was 6.0 × 10-8g / mL (3σ). The relative standard deviation (RSD) was 1.3% (n = 10). The method has been applied to the determination of paracetamol in acetaminophen tablets with the recoveries of 94.5% -105.0%.