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采用聚丙烯酰胺凝胶电泳法检测伤寒杆菌和甲型副伤寒杆菌的 CWDMs及其亲代细菌型和伤寒杆菌粗糙型的乳酸脱氢酶 ( L DH)同功酶 ,以了解沙门菌 CWDMs生物氧化的特点和机制 ,探讨 CWDMs变异的性质。结果表明 ,伤寒杆菌和甲型副伤寒杆菌的细菌型及伤寒杆菌粗糙型在聚丙烯酰胺凝胶电泳后显示出相同的 4种具有不同泳动速率的 L DH同功酶 ,但 CWDMs仅显示 2种 L DH。CWDMs的 2种 L DH同功酶与其亲代细菌型及伤寒杆菌粗糙型的 2种快泳动的 L DH相同 ,但缺乏慢泳动的 2种 L DH同功酶。提示 CWDMs不能酵解糖类以及只能在有氧环境中利用丙酮酸生长繁殖的特性可能与其丧失了 2种慢泳动的 L DH同功酶有关
The CWDMs of Salmonella typhi and Paratyphoid paratyphi A, their parental bacterium and the rough type lactate dehydrogenase (L DH) isoenzyme of Salmonella typhi were detected by polyacrylamide gel electrophoresis to understand the biological oxidation of Salmonella CWDMs Characteristics and mechanisms to explore the nature of CWDMs variation. The results showed that the bacterium type and Salmonella typhi pathogenicity of Salmonella typhi and Salmonella paratyphi A showed the same L DH isozymes with different rates of motility after polyacrylamide gel electrophoresis but CWDMs showed only 2 L DH. The two L DH isoenzymes of CWDMs were the same as their parental and Salmonella typhimurium two rough-moving L DHs, but lacking two L DH isoenzymes slowly moving. Suggesting that CWDMs could not ferment carbohydrates and the trait of pyruvate growth and reproduction in aerobic environment may be related to the loss of two slow-moving L DH isoenzymes