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目的探讨beclin1在乳腺癌中的可能下调机制。方法用Real-time RT-PCR检测34例乳腺癌中be-clin1 mRNA的表达;Q-PCR分析beclin1是否存在基因的缺失;亚硫酸氢钠测序法检测beclin1基因启动子区域的CpG岛甲基化。结果乳腺癌组织中beclin1的mRNA表达水平与癌旁组织比较显著下调(P=0.005);Q-PCR发现62%的肿瘤标本中beclin1基因存在缺失;在6例乳腺癌mRNA表达下调的乳腺癌标本中发现启动子区域异常的DNA甲基化。结论beclin1基因的缺失和启动子区域的异常甲基化可能是其在肿瘤细胞中失活的两种机制。
Objective To investigate the possible mechanism of beclin1 in breast cancer. Methods Real-time RT-PCR was used to detect the expression of be-clin1 mRNA in 34 cases of breast cancer. The deletion of beclin1 gene was detected by Q-PCR. The methylation of CpG island in promoter region of beclin1 gene was detected by sodium bisulfite sequencing . Results The mRNA expression of beclin1 in breast cancer tissues was significantly down-regulated compared with that in adjacent non-cancerous tissues (P = 0.005). There was a loss of beclin1 gene in 62% of the tumor samples by Q-PCR. In 6 breast cancer specimens with down-regulated mRNA expression, Aberrant DNA methylation in the promoter region was found. Conclusion The deletion of beclin1 gene and abnormal methylation of the promoter region may be two mechanisms of its inactivation in tumor cells.