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为了研究金丝桃甙(Hyp)脊髓镇痛作用及机制。采用在小鼠温浴浸尾和甲醛致痛模型上,分别观察鞘内给药后小鼠甩尾反应潜伏期和疼痛评分的改变,新生鼠脑细胞游离钙用Fura—2/AM法测定。结果显示,Hyp(5.0、2.5mg·kg~(-1))显著延长小鼠甩尾反应潜伏期,并呈一定量效关系;Hyp还可抑制甲醛致小鼠疼痛,并降低疼痛评分,EGTA和CaCL_2可分别加强和拮抗Hyp(2.5mg·kg~(-1))镇痛;进一步研究发现Hyp可抑制KCI诱导的分离的新生大鼠脑细胞内[ca~(2+)]的增高。由此可见,Hyp有脊髓镇痛作用,其机制可能与抑制钙内流有关。
In order to study the analgesic effect and mechanism of hyperglycemia (Hyp) in the spinal cord. The changes in latency and pain scores of tail-flick responses in mice after intrathecal administration were observed in a mouse model of warm bath-immersion and formaldehyde-induced pain. The free calcium in neonatal rat brain cells was measured by Fura-2/AM method. The results showed that Hyp (5.0, 2.5 mg·kg -1) significantly prolonged the latency of tail flick reaction in mice and showed a certain dose-effect relationship; Hyp also inhibited formaldehyde-induced pain in mice and reduced the pain score, EGTA and CaCL 2 can enhance and antagonize the analgesia of Hyp (2.5 mg·kg -1), respectively. Further study found that Hyp can inhibit the increase of [ca 2+ ] in the isolated rat brain cells induced by KCI. This shows that Hyp has spinal analgesic effect and its mechanism may be related to inhibition of calcium influx.