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笔者经过几年实践,证实了利用蜂窝煤炉形成的干热无菌区,在无菌区内接种不论生产哪级菌种都方便易行,简单可靠,只要掌握好操作要领成功率可达99%以上。现将具体操作简述如下。(一)用具及材料蜂窝煤炉一个,接种针、铲、护筒(用铁皮做成长20cm 直径与原种瓶外径相当的筒)、刀片、绳子、0.1%升汞棉球。如接菌袋单人操作需增设一个平台(与炉子齐高的方凳即可),蜂窝煤球。
The author after several years of practice, confirmed the use of briquette furnace to form a dry heat sterile area, regardless of the level of bacteria inoculation in the aseptic area are easy to operate, simple and reliable, as long as the master operation of the successful rate of up to 99% the above. Now the specific operation is as follows. (A) Equipment and materials A honeycomb furnace, inoculation of needles, shovels, protective tube (made of metal 20cm in diameter and diameter of the original bottle diameter tube), blades, rope, 0.1% mercurial cotton balls. Such as inoculation bags need to set up a single operation platform (with the stove altogether square stool can), briquettes briquettes.