体外培养神经细胞是神经生物学中研究神经细胞发育再生的一种重要手段。但胆碱乙酰化酶（ＣｈＡＴ）免疫组化法价格昂贵，我们采用乙酰胆碱酯酶（ＡｃｈＥ）组化方法同样能清晰显示胆碱能神经元的生长发育情况。本文对体外培养神经细胞的ＡｃｈＥ组化染色的方法要点，时间控制及其原理进行了讨论。并与鼠脑切片标本ＡｃｈＥ组化法作比较分析 In vitro culture of nerve cells in neurobiology is an important means of studying the development and regeneration of nerve cells. However, ChAT immunohistochemistry is expensive, and we used AchE staining to clearly show the growth and development of cholinergic neurons. In this paper, the main points, time control and principle of AchE staining of cultured nerve cells in vitro were discussed. And compared with the rat brain sections AchE histochemical method for comparative analysis