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目的研究纤维蛋白原(Fg)Bβ启动子区-455G/A、-854G/A、-1420G/A3个单核苷酸多态性(SNP)与缺血性脑卒中(IS)的关系。方法应用病例对照分析和聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法,对160例IS患者与130名健康对照组的FgBβ-455G/A、-854G/A、-1420G/A等位基因频率和基因型频率与IS之间的关系进行分析。结果IS组的FgBβ-455G/A SNP A位点的等位基因频率为0·3688,与对照组的A位点的等位基因频率0·2538差异有统计学意义(P<0·01),IS组-455位点的基因型频率(GG:33·8%,GA:58·8%,AA:7·5%)与对照组的基因型频率(GG:55·4%,GA:38·5%,AA:6·2%)差异有统计学意义(P=0·01),IS组中-455位点的GA+AA基因型频率(66·3%)高于对照组的GA+AA频率(44·6%,P=0·001);在高血压分层分析后-455位点的A等位基因频率和GA+AA基因型频率在病例组和对照组之间差异有统计学意义,在两组间-854G/A、-1420G/A的基因型和等位基因频率的差异无统计学意义。结论FgBβ-455G/A位点与IS有关联,可能是IS的独立危险因素,-854G/A、-1420G/A与IS无关联。
Objective To investigate the relationship between -455G / A, -854G / A and -1420G / A3 single nucleotide polymorphisms (SNPs) in the Bβ promoter region of fibrinogen (Fg) and ischemic stroke (IS). METHODS: FgBβ-455G / A, -854G / A, -1420G (superscript +) and mRG-1 were detected in 160 patients with IS and 130 healthy controls by case-control analysis and polymerase chain reaction- restriction fragment length polymorphism (PCR-RFLP) / A allele frequency and the relationship between genotype frequency and IS were analyzed. Results The allele frequency of FgBβ-455G / A SNP A locus in IS group was 0.3688, which was significantly different from that of control group at locus A 02575 (P <0.01) (GG: 55.4%, GA: 58.8%, AA: 7.5%) in the IS group were higher than those in the control group The difference was statistically significant (P = 0.01). The frequency of GA + AA genotype at -455 in IS group (66.3%) was higher than that in control group GA + AA frequency (44.6%, P = 0.001). The frequency of A allele and the frequency of GA + AA genotype at -455 after stratified hypertension were different between the case group and the control group Statistically significant difference was not found between genotype and allele frequencies of -854G / A and -1420G / A between the two groups. Conclusion FgBβ-455G / A locus is associated with IS, which may be an independent risk factor for IS. There is no correlation between -854G / A and -1420G / A and IS.