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目的 研究电离辐射对离体培养的兔晶体上皮细胞中酸性磷酸酯酶的影响。方法 用6 0Coγ线单次照射培养的第三代兔晶体上皮细胞 ,经ACP细胞化学方法染色后 ,用图像分析技术对其进行定量分析。结果 1、晶体上皮细胞于培养后 3天可见有单个细胞从晶体上皮长出 ,至培养第三代的细胞经 30Gy6 0 Coγ线照射后 ,细胞生长速度变慢 ,细胞形态不规则。照射后ACP呈强阳性表达 ,照后 7天更为明显。电镜观察可见 ,晶体上皮细胞核仁肿胀 ,染色质稀少 ,内质网扩张 ,线粒体萎缩。溶酶体膜破裂 ,随着照后时间延长 ,病变更为明显。 2、定量结果显示 :各剂量组在照后3和 7天 ,ACP的面积、平均光密度、积分光密度均显著高于对照组 (P <0 .0 0 1) ,其中 7天各指标升高更为明显 ,此结果与电镜所见相一致。结果提示 ,电离辐射对细胞溶酶体膜造成损伤是ACP活力改变可能的机理 ,而后者可能是导致晶体上皮损伤和死亡的原因之一。
Objective To study the effect of ionizing radiation on acid phosphatase in cultured rabbit lens epithelial cells. Methods The third generation of cultured rabbit lens epithelial cells were irradiated with 60Co γ ray in a single irradiation. The cells were stained with ACP cytochemistry and quantified by image analysis. The results showed that single cell grew out of the crystal epithelium 3 days after culture, and the cell growth rate became slower and the cell shape was irregular after the third generation of cells were irradiated by 30 Gy 60 Coγ ray. After irradiation, ACP showed a strong positive expression, 7 days after irradiation was more obvious. Electron microscopy shows that the lens epithelial cell nucleolus swelling, rare chromatin, endoplasmic reticulum dilatation, mitochondria atrophy. Lysosomal membrane rupture, with the extension of time after irradiation, lesions become more apparent. Quantitative results showed that the area, the average optical density and the integral optical density of ACP were significantly higher than those of the control group (P <0.01) at 3 and 7 days after irradiation High more obvious, this result is consistent with the electron microscope. The results suggest that ionizing radiation damage to the cell lysosomal membrane is a possible mechanism of ACP activity changes, which may be one of the reasons leading to crystal epithelial damage and death.