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[Objective] To determine the content of quercetin-rhamnoside in different medicinal parts of Acanthopanax senticosus (Rupr. et Maxim.) Harms and compare them, so as to provide scientific basis for rational utilization and quality evaluation of A. senticosus. [Method] HPLC was used to determine the content of quercetin-rhamnoside in root, stem, leaf, flower and fruit. HPLC analysis was performed on Dalian Jiangshen Nucleosil C18 column (4.6 mm×150 mm, 5 μm) with mobile phase of methyl alcohol-water (V/V, 40∶ 60) and flow rate of 1.1 mL/min. The other conditions were: detection wavelength of 370 nm, column temperature of 30 ℃, sampling amount of 10 μL. [Result] Different medicinal parts of A. senticosus were arranged in order of quercetin-rhamnoside content: flower buds > leaves > fruits > stems > roots. The content of quercetin-rhamnoside was the highest in flower buds (4.43%), followed by leaves (0.61%), and the least in roots (0.01%). [Conclusion] This method is simple, quick and accurate, and can be used for analysis of quercetin-rhamnoside in different medicinal parts of A. senticosus.