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目的通过给予大鼠伸筋草提取物,探讨其中主要活性成分玉柏碱的药动学特征。方法利用RP-HPLC法,以Centurysil C18EPS为色谱柱,甲醇-水(61∶39)为流动相;紫外检测器测定大鼠ig伸筋草提取物后血浆中玉柏碱的血药浓度,计算药动学参数。结果玉柏碱在大鼠体内呈二室模型分布,线性范围为0.354~14.16μg/mL,r=0.999 8。日内和日间精密度均小于5%。玉柏碱口服给药后在大鼠体内的主要药动学参数为:t1/2为2.681h,tmax为0.75h,Cmax为6.309mg/L,AUC(0→t)为16.626mg/L,AUC(0→∞)为18.798mg/L。结论本方法适用于大鼠血浆中玉柏碱的检测及其体内药动学研究。
OBJECTIVE To investigate the pharmacokinetic characteristics of jade alkaloids, the main active ingredient of which, Methods RP-HPLC was performed on a Centurysil C18EPS column with methanol-water (61:39) as the mobile phase. The plasma concentration of libeributerol Dynamic parameters. Results The results showed that there was a two-compartment model distribution in rats. The linear range was 0.354-14.14 μg / mL, r = 0.999 8. Intra- and inter-day precision are less than 5%. The main pharmacokinetic parameters in rats after oral administration were as follows: t1 / 2 was 2.681h, tmax was 0.75h, Cmax was 6.309mg / L, AUC (0 → t) was 16.626mg / L, AUC (0 → ∞) was 18.798 mg / L. Conclusion This method is suitable for the determination of jade alkaloids in rat plasma and its in vivo pharmacokinetics.