论文部分内容阅读
目的研究草乌甲素(BLA)对小鼠腹腔巨噬细胞趋化功能的影响。方法小鼠腹腔巨噬细胞经分离后,制成细胞密度为1×10~6个·mL~(-1)的细胞悬液;而趋化液由4部分按不同比例组成,即细胞培养基RPMI-1640液,溶剂(0.01%DMSO),BLA溶液(质量浓度分别为80、40、20、10、5μg·L~(-1))和趋化因子(MCP-1或补体激活产物)。应用transwell 24孔趋化板检测腹腔巨噬细胞在不同趋化液中的趋化功能,以评价BLA对不同条件下细胞趋化作用的影响。结果 MCP-1和补体激活产物均可使腹腔巨噬细胞的趋化指数显著高于溶剂对照组(P<0.01),而该趋化指数可受到BLA溶液的抑制(P<0.01)。但单用BLA(80、40、20、10、5μg·L~(-1))对腹腔巨噬细胞的趋化指数与溶剂对照组相似,差异均无统计学意义(P>0.05)。结论 BLA能抑制趋化因子对腹腔巨噬细胞的趋化作用,但对腹腔巨噬细胞无直接作用。
Objective To study the effect of abalone (BLA) on the chemotactic function of murine peritoneal macrophages. Methods Mouse peritoneal macrophages were separated and the cell suspension with cell density of 1 × 10 ~ 6 · mL ~ (-1) was prepared. The chemotactic fluid consisted of 4 parts in different proportions, namely cell culture medium RPMI-1640 solution, solvent (0.01% DMSO), BLA solution (concentration of 80, 40, 20, 10, 5μg · L -1) and chemokine (MCP-1 or complement activation product). The chemotactic function of peritoneal macrophages in different chemotactic fluids was assayed by transwell 24-well chemotaxis assay to evaluate the effect of BLA on chemotaxis of cells under different conditions. Results The chemotactic index of peritoneal macrophages was significantly higher than that of solvent control group (P <0.01), and the chemotactic index was inhibited by BLA solution (P <0.01). However, the chemotactic index of peritoneal macrophages with BLA alone (80, 40, 20, 10, 5μg · L -1) was similar to that of the solvent control group, with no significant difference (P> 0.05). Conclusion BLA can inhibit the chemotactic effect of chemokines on peritoneal macrophages, but has no direct effect on peritoneal macrophages.