论文部分内容阅读
观察了分离的人外周血树突状细胞分别与同种异体淋巴细胞和淋巴因子激活杀伤细胞于37℃,5% CO_2,饱湿条件下共育形成的细胞簇,以4～25个细胞聚集计为一簇进行活细胞计数. 结果发现:经16小时培养的树突状细胞,共育24小时比共育4小时形成的细胞簇明显增加,而与共育28小时者无明显差异:培养36小时的树突状细胞与培养16小时的树突状细胞相比,共育4小时的细胞簇相应增加;培养液内加入淋巴因子激活的杀伤细胞培养上清液,细胞簇也相应增多.以上结果表明,人血树突状细胞与淋巴细胞或淋巴因子激活的杀伤细胞形成细胞簇与树突状细胞单独培养的时间、树突状细胞与淋巴细胞或淋巴因子激活的杀伤细胞共育的时间均有一定的关系.淋巴因子激活的杀伤细胞培养上清液对细胞簇形成也有一定促进作用.
The isolated dendritic cells from human peripheral blood were incubated with allogeneic lymphocytes and lymphokine-activated killer cells in 37 ℃, 5% CO 2 and saturating conditions for 4 ~ 25 cell aggregation Counted as a cluster of live cells count.The results showed that: 16 hours after incubation of dendritic cells, a total of 24 hours after co-cultured 4 hours formed a significant increase in cell clusters, and 28 hours co-incubation were no significant difference: culture 36 Hours of dendritic cells and cultured 16 hours of dendritic cells compared to a total of 4 hours of co-cultured cell clusters increased; the culture fluid was added lymphokine-activated killer cell culture supernatant, cell clusters also increased accordingly. The results show that human dendritic cells and lymphocytes or lymphokine-activated killer cells form clusters of cells and dendritic cells cultured alone for a period of time between dendritic cells and lymphocytes or lymphokine-activated killer cells Have a certain relationship.Lymphokine-activated killer cell culture supernatant also contribute to the formation of cell clusters.