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目的分析4种自身抗原与狼疮性肾炎(LN)的相关性,以寻找具有更高敏感性与特异性的特征性自身抗原。方法收集广西医科大学第一附属医院检验科2013年7月—2014年7月期间的患者血清120例,其中正常人血清、系统性红斑狼疮(SLE)患者血清、LN患者血清及肾病综合征(NS)患者血清各30例。以Cr ELISA法检测四种阳性抗原蛋白在各组血清中的表达情况,并以生物信息学分析结果。结果 A1、A2在SLE组中的表达量(0.67±0.36)、(0.31±0.18)相对在正常人组中的表达量(0.47±0.24)、(0.22±0.10)与NS组中的表达量(0.33±0.17)、(0.19±0.10)更高(P<0.05),与LN组中的表达量(0.53±0.27)、(0.27±0.14),差异无统计学意义(P>0.05)。结论 4种抗原中,A1、A2的敏感性相对较高,更可能是SLE/LN的特征性自身抗原,有望成为SLE/LN的诊断依据。
Objective To analyze the correlation between four autoantigens and lupus nephritis (LN) in order to find a characteristic autoantigen with higher sensitivity and specificity. Methods A total of 120 patients with serum from January 2013 to July 2014 were collected from Department of Laboratory Medicine, the First Affiliated Hospital of Guangxi Medical University. Serum from patients with systemic lupus erythematosus (SLE), serum from patients with LN and nephrotic syndrome NS) patients with 30 cases of serum. The expression of four positive antigen proteins in serum of each group was detected by Cr ELISA, and the results were analyzed by bioinformatics. Results The expression levels of A1 and A2 in SLE group (0.67 ± 0.36) and (0.31 ± 0.18) vs normal group (0.47 ± 0.24), (0.22 ± 0.10) and NS group 0.33 ± 0.17), (0.19 ± 0.10) and (0.33 ± 0.27) and (0.27 ± 0.14) respectively. There was no significant difference between them and LN group (P> 0.05). Conclusion Among the four antigens, the sensitivity of A1 and A2 is relatively high, which is more likely to be the characteristic autoantigen of SLE / LN, which is expected to become the diagnostic basis of SLE / LN.