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目的:通过变应性鼻炎动物模型了解Eotaxin在变应性鼻炎中的表达以及变态反应炎性递质组胺对Eotaxin表达的影响,以期深入了解变应性鼻炎的发病机制。方法:卵蛋白致敏法建立变应性鼻炎大鼠模型,应用免疫组织化学、RT-PCR及ELISA法,观察组胺及组胺HR1拮抗剂干预后鼻黏膜、血清及鼻腔灌洗液中Eotaxin的表达量或含量变化。结果:变应性鼻炎干预组与变应性鼻炎无干预组相比,鼻腔灌洗液以及鼻黏膜中Eotaxin的表达量较前者显著增加(P<0.05),而H1拮抗剂干预组血清、鼻腔灌洗液以及鼻黏膜中Eotaxin增加均显著受到抑制(P<0.05)。结论:本实验进一步确认了Eotaxin参与了变应性鼻炎发病的理论基础。组胺作为一种变应性鼻炎中的重要炎症递质可以通过影响Eotaxin的表达参与变应性鼻炎的发病过程,且HR1亦参与了这一过程。
OBJECTIVE: To investigate the expression of Eotaxin in allergic rhinitis and the effect of allergic neurotransmitter histamine on Eotaxin expression in an animal model of allergic rhinitis, in order to understand the pathogenesis of allergic rhinitis. Methods: The model of allergic rhinitis was established by ovalbumin sensitization. Immunohistochemistry, RT-PCR and ELISA were used to observe the expression of Eotaxin in nasal mucosa, serum and nasal lavage after intervention of histamine and histamine HR1 antagonist Of the expression or content changes. Results: The expression of Eotaxin in nasal lavage fluid and nasal mucosa of allergic rhinitis intervention group was significantly higher than that of the control group (P <0.05), while the serum levels of nasal cavity Laxation fluid and Eotaxin in nasal mucosa were significantly inhibited (P <0.05). Conclusion: This experiment further confirmed that Eotaxin is involved in the pathogenesis of allergic rhinitis. Histamine as an important inflammatory neurotransmitter in allergic rhinitis can participate in the pathogenesis of allergic rhinitis by affecting the expression of Eotaxin, and HR1 is also involved in this process.