论文部分内容阅读
目的 :为了解本地区原发性肝癌 (PHC)中HGV感染状况和与输血的关系。方法 :采用逆转录 套式聚合酸链反应法 (RT nPCR)对 2 0例PHC和 4 4例职业献血员进行了HGV RNA检测 ,并对 3例阳性产物纯化克隆后 ,采用ABIPrism 377DNA自动测序仪 ,对其 5′非编码区基因序列 ( 115～ 4 67nt)进行了测定。结果 :2 0例PHC中 ,2例 ( 10 .0 % )HGV RNA阳性 ,该 2例分别为HBV、HCV、HGV三重感染和HBV、HGV重叠感染 ,且均有输血史 ;4 4例职业献血员中 ,1例 ( 2 3% )HGV RNA阳性。该 3株HGV核苷酸序列同源性为 97.3%～ 98.8% ,与GBV C(U36380 )、HGV (U4 4 40 2 )和HGV3型 (D90 60 1)的核苷酸同源性分别为 88.3%～ 89.4 %、88.4 %～ 89.7%和 95.2 %～ 96.3%。结论 :PHC中HGV感染与输血有关 ;HGV可能与PHC的发生无关。本地区HGV感染株属基因 3型
Objective: To understand the relationship between HGV infection and transfusion in primary liver cancer (PHC) in this area. Methods: HGV RNA was detected by reverse transcription polymerase chain reaction (RT nPCR) in twenty cases of PHC and forty four blood donors. The positive clones were purified by ABIPrism 377 DNA sequencer , And its 5 ’non-coding region gene sequence (115 ~ 4 67nt) was measured. Results: 2 cases (10.0%) had positive HGV RNA in 20 cases of PHC. The 2 cases were HBV, HCV, HGV triple infection and HBV, HGV overlap infection, and had a history of blood transfusion; 44 cases of blood donation One (23%) HGV RNA positive patients. The homologies of the three HGV nucleotide sequences were 97.3% ~ 98.8%. The nucleotide homology with the nucleotide sequences of GBV C (U36380), HGV (U4 4 40 2) and HGV3 (D90 60 1) were 88.3 % To 89.4%, 88.4% to 89.7% and 95.2% to 96.3%. Conclusion: HGV infection in PHC is related to blood transfusion; HGV may not be related to PHC. HGV infection in this region genotype 3