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上海市某幼儿园 2 0 0 0年 10～ 11月发生手足口病 (HFMD)流行 ,从患儿粪便标本中分离到 13株肠道病毒 71型 (EV71) ,血清学实验证明 ,所分离的病毒为此次HFMD流行的病因。对其中的 4株病毒 (SHANGHAI EV71 H2 5、SHANGHAI EV71 H2 6、SHANGHAI EV71 F1和SHANGHAI EV71 F2 )进行了VP1区全基因核苷酸序列测定 ,并做了比较分析。 4株EV71VP1区全基因均为 891个核苷酸 ,编码 2 97个氨基酸 ,与检索到的广东省深圳市和台湾省HFMD病人EV71分离株同源性较高 (>94 % ) ,与脑炎EV71分离株 (BrCr株 )同源性低 (<83% )。种系发生树分析亦表明 ,上海市的 4株EV71与深圳株 (AF30 2 996 EV71 SHZH98)和台湾株 (AF2 86 5 31 FV71 TW 98)亲缘关系较近 ,与BrCr株亲缘关系较远。因此上海市HFMD 4株EV71分离株为本地流行株。
HFMD epidemics occurred in a kindergarten in Shanghai from October to November in 2010 and 13 strains of enterovirus 71 (EV71) were isolated from stool samples of children. Serological tests showed that the isolated virus The etiology of the epidemic of HFMD. The nucleotide sequences of VP1 gene of four strains of viruses (SHANGHAI EV71 H2 5, SHANGHAI EV71 H2 6, SHANGHAI EV71 F1 and SHANGHAI EV71 F2) were determined and compared. The four strains of EV71VP1 were 891 nucleotides in length encoding a protein of 2 97 amino acids with high homology (> 94%) with the retrieved EV71 isolates of HFMD patients in Shenzhen and Taiwan provinces of Guangdong Province and encephalitis The EV71 isolate (BrCr strain) has low homology (<83%). Phylogenetic tree analysis also showed that 4 EV71 strains in Shanghai were closely related to the Shenzhen strain (AF30 2 996 EV71 SHZH98) and Taiwan strain (AF2 86 5 31 FV71 TW 98), and were more distantly related to the BrCr strain. Therefore, four Shanghai strains of HFMD EV71 isolates were endemic strains.