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目的 通过添加O-羧甲基壳聚糖(oxygen-carboxymethylated chitosan,O-CMC)和庆大霉素对硫酸钙骨水泥(calcium sulfate cement,CSC)进行改性,提高其在骨修复领域应用的可行性.方法 通过在CSC液相中添加不同浓度O-CMC(0.1wt％、0.3wt％、0.5wt％、0.7wt％、1.0wt％),制备O-CMC/CSC.通过可注射性、抗压强度、降解率、模拟体液pH值及Ca2+浓度测试、成分与形貌表征观察以及细胞毒性测试,分析O-CMC浓度对CSC在可注射性、抗压强度、降解行为及成骨活性方面的影响.综合以上检测指标,确定最佳性能的O-CMC/CSC,并以此复合不同浓度庆大霉素(0.5wt％、1.5wt％、2.5wt％),探究其抗压强度和抗菌性.结果 O-CMC/CSC可注射时间均超过5 min,且随O-CMC浓度的增加而显著延长(P＜0.05).改良后的骨水泥抗压强度介于11 ～ 18 MPa之间,0.5wt％O-CMC/CSC抗压强度最高(P＜0.05).O-CMC/CSC降解速率随O-CMC浓度增加而呈减小趋势,但组间比较差异无统计学意义(P＞0.05).浸泡液pH值变化在7.2～7.4之间,Ca2+浓度稳定在6 ～8 mmol/L.成分与形貌表征观察显示,相比于CSC,O-CMC/CSC在模拟体液中的诱导矿化能力更强,具有更好生物活性.综合以上检测指标结果,确定0.5wt％O-CMC/CSC性能最佳,加入庆大霉素后其抗压强度均大于5 MPa且具有抗菌性.结论 O-CMC能够有效提高CSC的可注射性、抗压强度和成骨活性,加入庆大霉素后复合骨水泥具有抗菌性.“,”Objective To extend its application in the field ofbone repair by adding oxygen-carboxymethylated chitosan (O-CMC) and gentamicin for modification of the calcium sulfate cement (CSC).Methods The O-CMC/CSC was prepared by adding O-CMC with different concentrations (0.1wt％,0.3wt％,0.5wt％,0.7wt％,and 1.0wt％) in the CSC liquid phase.The effect of O-CMC on the CSC was evaluated by testing the injectability,compressive strength,degradation rate,pH value,cytotoxicity and osteogenesis.After the optimal concentration of O-CMC was determined,gentamicin with different concentrations (0.5wt％,1.5wt％,and 2.5wt％) was added in the O-CMC/CSC,and then the compressive strength and antibacterial properties were investigated.Results After adding O-CMC in the CSC liquid phase,the injection time of O-CMC/CSC was increased to more than 5 minutes;it significantly prolonged with increased concentration of O-CMC (P＜0.05).The compressive strength of the modified bone cement was in the range of 11-18 MPa and it was the highest when the concentration of O-CMC was 0.5wt％ (P＜0.05).The degradation rate of O-CMC/CSC was not influenced obviously by O-CMC (P＞0.05).The pH value was in the range of 7.2-7.4 and Ca2+ concentration was in the range of 6-8 mmol/L.In vitro mineralization experiment indicated that the induced mineralization ability of O-CMC/CSC was much higher than that of pure CSC.The 0.5wt％ O-CMC/CSC had the best performance;the compressive strength ofthe composite bone cement was above 5 MPa after gentamicin was added,which had antibacterial effect.Conclusion O-CMC is able to effectively improve the injection,compressive strength,and osteogenic activity of CSC;in addition,antibacterial properties is obtained in the CSC after adding gentamicin.