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从感染彭亨丝虫和马来丝虫的猫采血(每毫升血加10单位肝素)收集微丝蚴,从感染的棉鼠收集棉鼠丝虫微丝蚴。感染班氏丝虫微丝蚴的血来自坦桑尼亚(1973年起保存于液氮内)。用5微米核孔薄膜滤过器分离微丝蚴,洗涤滤膜,将微丝蚴混悬于 pH 7. 4的Hanks 平衡盐液(HBSS)中。分别将微丝蚴放在含胃蛋白酶、胰蛋白酶、糜蛋白酶、羧肽酶-A、淀粉糖甙酶、甲壳酶、胶原酶、透明质酸酶、神经氨酸酶、肽链内断酶、亮氨酸氨基肽
Microfilars were collected from cats infected with Paenibacillar filaments and Malayi worms (microfilament of 10 units per ml of blood plus 10 units of heparin), and microfilariae from the infected cotton rats were collected. Infection with Bancroftian filarial microfilaria from Tanzania (preserved in liquid nitrogen since 1973). Microfilars were separated using a 5 micron nuclear membrane filter and the filters were washed and the microfilariae were suspended in Hanks Balanced Salt Solution (HBSS) pH 7.4. The microfilariae were respectively treated with pepsin, trypsin, chymotrypsin, carboxypeptidase-A, amylosidase, chitinase, collagenase, hyaluronidase, neuraminidase, Leucine amino peptide