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目的 探讨Tbx3基因在乳腺癌发生发展过程中的作用。方法 收取新鲜乳腺癌和癌旁乳腺组织各44例,以Tripure试剂提取细胞总RNA,以RT PCR法检测两种组织中Tbx3基因的表达水平,并比较其表达状况与乳腺癌相关临床病理因素之间的关系。结果 Tbx3基因在乳腺癌组织中的表达显著强于癌旁乳腺组织(P<0 01),与患者的年龄、肿瘤大小无关,但随着临床分期的进展、出现腋窝淋巴结转移、肿瘤浸润程度增加和组织学级别的增高,该基因的表达明显增强。结论 高表达的Tbx3基因可能在乳腺癌发生发展过程中起促进作用,并可能是乳腺癌进展中关键性转变的标志性基因之一。
Objective To investigate the role of Tbx3 gene in the development of breast cancer. Methods The total RNA was extracted from fresh breast cancer and adjacent normal breast tissue by Tripure reagent. The expression of Tbx3 gene in both tissues was detected by RT-PCR. The expression of Tbx3 gene was compared with the clinicopathological factors Relationship between. Results The expression of Tbx3 gene in breast cancer tissues was significantly higher than that in adjacent noncancerous tissues (P <0.01), but not with age and tumor size. However, as the clinical stage progressed, axillary lymph node metastasis and tumor infiltration increased And histological grade increased, the expression of the gene was significantly enhanced. Conclusion High expression of Tbx3 gene may play a role in the development of breast cancer and may be one of the key genes in the progression of breast cancer.