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目的观察小鼠成长过程中肝肾小肠AKP活性变化规律及其与各器官的功能成熟关系。方法采用比色法、原位复性电泳法对出生后D1、D7、D14、D21和Da小鼠肝、肾、小肠AKP活性进行了分析。结果比色结果表明:小鼠肝、肾、小肠中AKP活性表现为:小肠﹥肾﹥肝,小肠中的AKP活性显著高于肝和肾中的AKP活性。在小鼠成长过程中,肝和小肠内AKP活性均呈现先上升后下降的规律,肾内AKP活性总体呈上升趋势。电泳结果表明:在小鼠成长发育过程中肝、肾和小肠中均出现了2种AKP同工酶,各同工酶活性随着小鼠的生长发育发生一定的变化。结论 AKP活性变化可能与小鼠各器官的功能成熟与完善有一定关系。
Objective To observe the changes of AKP activity in hepatorenal small intestine and its relationship with functional maturation of various organs during the growth of mice. Methods The activity of AKP in the liver, kidney and small intestine of D1, D7, D14, D21 and Da mice after birth was analyzed by colorimetric method and in situ renaturing electrophoresis. Results The results of colorimetric assay showed that the activities of AKP in liver, kidney and small intestine were as follows: small intestine> kidney> liver, AKP activity in small intestine was significantly higher than that in liver and kidney. During the process of mouse growth, the activity of AKP in liver and small intestine first increased and then decreased, and the activity of AKP in the kidney showed an overall upward trend. The results of electrophoresis showed that there were two kinds of AKP isoenzymes in the liver, kidney and small intestine during the growth and development of mice. The isoenzyme activity varied with the growth and development of mice. Conclusion The changes of AKP activity may be related to the function maturation and perfection of various organs in mice.