拮抗细菌对6种植物病原菌的抑菌作用

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分别就拮抗细菌生防菌2[短小芽胞杆菌(Bacilluspumilus)]和生防菌1[枯草芽孢杆菌(Bacillussubtilis)]对稻瘟病菌(Magnaporthegrisea)、芦笋茎枯病菌(Phomopsisiasparagi)、尖镰刀菌(Fusariumoxysporum)、玉米小斑菌(Bipolariamaydis)、百合灰霉病菌(Botrytiselliptica)和青枯病菌(Ralstoniasolanacearum)6种植物病原菌的抑制作用进行了测定,结果发现,生防菌2发酵液50倍稀释液对稻瘟病菌分生孢子萌发的抑制率为62 6%,100倍稀释液则为55 3%,对分生孢子附着胞形成的抑制作用不明显;将稻瘟病菌和生防菌2发酵液同时接入燕麦培养基中,生防菌2对稻瘟病菌菌落的生长有明显的抑制作用;生防菌2对芦笋茎枯病菌、尖镰刀菌、玉米小斑菌和百合灰霉病菌分生孢子萌发的抑制作用不明显;生防菌1对青枯病菌生长有明显的抑制作用,而生防菌2对青枯菌抑制作用不明显。 Bacillus pumilus and Bacillus subtilis were tested against Magnaporthe grisea, Phomopsis paraspai, Fusarium oxysporum, ), Bipolariamaydis, Botrytis iliptica and Ralstoniasola nacearum were studied. The results showed that the 50-fold dilution of biocontrol bacterium 2 fermentation broth against rice The inhibition rate of conidia germination was 62 6%, while that of 100-fold dilution was 55 3%. The inhibitory effect on conidia formation was insignificant. At the same time, In oat medium, biocontrol bacteria 2 had a significant inhibitory effect on the growth of Magnaporthe grisea colonies. Biocontrol agent 2 had no effect on the spore germination of Asparagus officinalis, Fusarium oxysporum, Of the inhibition was not obvious; biocontrol bacteria 1 had a significant inhibitory effect on the growth of bacterial wilt, while biocontrol bacteria 2 had no obvious inhibitory effect on R. solanacearum.
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