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目的建立红景天苷含量超高效液相色谱测定方法,并应用于红景天苷在大鼠体内的药代动力学研究。方法采用Waters ACQUITY UPLC H-CLASS T3色谱柱(50mm×2.1mm,1.8 m),流动相:乙腈/水(1/9),流速:0.25 ml/min,检测波长为280 nm,进样量5 l,内标为4-乙酰氨基酚。健康大鼠尾静脉注射给药后,在0.02、0.07、0.12、0.17、0.25、0.33、0.5、1.0、1.5、2 h时间点取血检测,并利用Kinetica软件对血药浓度-时间数据进行拟合。结果红景天苷质量浓度在1～400 g/ml范围内线性关系良好(r=0.9998),血浆中红景天苷的回收率≥90%,主要的药代动力学参数AUC(0-+∞)、T1/2、MRT、CL以及Vss分别为54.31±2.25 h/(mg/L)、0.65±0.04 h、0.77±0.03 h、0.37±0.02 L/h及0.28±0.02 L。结论本方法简单、快速、准确,适用于大鼠血浆中红景天苷的血药浓度测定及药代动力学研究。
AIM To establish a method for the determination of salidroside by ultra performance liquid chromatography (HPLC) and to study the pharmacokinetics of salidroside in rats. Methods Waters ACQUITY UPLC H-CLASS T3 (50 mm × 2.1 mm, 1.8 m) was used. The mobile phase was acetonitrile / water (1/9), the flow rate was 0.25 ml / min, the detection wavelength was 280 nm, l, the internal standard for 4-acetaminophen. Blood samples were taken from the tail vein of healthy rats at 0.02, 0.07, 0.12, 0.17, 0.25, 0.33, 0.5, 1.0, 1.5 and 2 h time points and the Kinetica software was used to analyze the plasma concentration- Together Results The salidroside had a good linearity (r = 0.9998) in the range of 1 ~ 400 g / ml, the salidroside recovery in plasma ≥ 90%, and the main pharmacokinetic parameters AUC (0- + ∞), T1 / 2, MRT, CL and Vss were 54.31 ± 2.25 h / (mg / L), 0.65 ± 0.04 h, 0.77 ± 0.03 h, 0.37 ± 0.02 L / h and 0.28 ± 0.02 L, respectively. Conclusion The method is simple, rapid and accurate and is suitable for the determination of plasma concentration of salidroside in rat plasma and its pharmacokinetics.