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建立了一种快速、简便的寡核苷酸链固定在普通玻璃介质上的ＤＮＡ 微矩阵并通过荧光检测的方法．首先将２种不同的寡核苷酸链固定在普通玻璃介质上，使寡核苷酸共价结合在经过处理的普通玻璃片表面形成点直径大约３ ｍ ｍ 的ＤＮＡ 微矩阵，然后先后分别与其对应互补的红黄不同颜色的荧光寡核苷酸链进行杂交实验．通过不同的波长激发，荧光显微镜下呈现红黄二色的点形成的矩阵．在固定的探针浓度为２ ｍ ｍ ｏｌ／Ｌ时，用１０ｐｍ ｏｌ／μＬ荧光标记Ｏｌｉｇｏ 进行杂交，出现阳性结果为１００％ ；固定的寡核苷酸浓度下限至１５．６ μｍ ｏｌ／Ｌ，通过ＳｃａｎｎｅｒＡｒｒａｙ ３０００可获得清晰的图象
A rapid and simple oligonucleotide chain immobilized on a common glass medium DNA microarray and through the fluorescence detection method. First, two different oligonucleotide chains were immobilized on a common glass medium, and the oligonucleotide was covalently bound to the surface of a normal glass plate to form a DNA microarray with a dot diameter of about 3 m. Then, Corresponding complementary red and yellow different fluorescent oligonucleotide chain hybridization experiments. Excited by different wavelengths, fluorescence microscopy shows a red, yellow and black dots form a matrix. At a fixed probe concentration of 2 m mol / L, hybridization with 10 pmol / μL fluorescently labeled Oligo yielded a 100% positive result; a fixed oligonucleotide concentration limit of 15.6 μm ol / L, With the ScannerArray 3000 you get a clear picture