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为了探索骨关节病在分子水平上的发病机理 ,观察了骨关节病的关节软骨细胞的癌基因表达及其相互关系。采用免疫组化方法 ,原位检测骨关节病关节软骨细胞的癌基因 (p5 3、p16、bcl- 2、erbB - 2 ,C -myc) ,以及增殖细胞核抗原 (PCNA)的表达 ,并观察其相互关系。结果发现 ,正常软骨细胞对数种癌基因呈弱阳性表达 ,骨关节病关节软骨细胞中的这些癌基因表达信号增强且阳性表达细胞数明显增多 ,erbB - 2和bcl- 2的阳性表达有同步关联。提示多种癌基因表达 ,可能与骨关节病的发病密切相关 ,但其中许多问题尚待进一步研究。
In order to explore the pathogenesis of osteoarthrosis at the molecular level, the oncogene expression and the relationship between articular chondrocytes of osteoarthrosis were observed. In situ immunohistochemistry was used to detect the expression of oncogenes (p53, p16, bcl-2, erbB-2, C-myc) and proliferating cell nuclear antigen (PCNA) in articular chondrocytes of osteoarthrosis. Relationships. The results showed that normal chondrocytes showed weak positive expression for several oncogenes, and the expression of these oncogenes in osteochondral articular chondrocytes increased and the number of positively expressed cells increased significantly, and the positive expression of erbB-2 and bcl-2 were synchronized. Associations. This suggests that many oncogene expressions may be closely related to the onset of osteoarthropathy, but many of these issues need further study.