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建立一种具有明确持久的门静脉压力升高、侧支循环形成又有典型、可靠的肝细胞损伤的门静脉高压症动物模型。方法本地健康犬12只随机分成实验组(6只)、对照组(6只)。实验组行门静脉主干缩窄1/2加丝线慢性栓塞并于术后1周后行四氯化碳腹腔注射;实验组行假手术及同样的CCL4腹腔注射方法。检测丙氨酸氨基转移酶(ALT)、天门冬酸转氨酶(AST)、总胆红素(TB)、凝血酶原时间(PT);术前和术后第20天各组食管下端平均血管数、平均血管口径、门静脉压力变化和肝脏组织病理学变化。结果实验组所有犬术后15天均出现腹壁静脉曲张,出现率100%;术后20天门静脉压力、食管下端平均血管数、平均血管口径实验组均较对照组增加(P<0.05);两组肝脏损伤评分均较术前有明显损害(P<0.05)但两组之间无明显差异。结论该模型门脉高压症明显、肝细胞损伤明确典型、实验终点明确、成功率高,是一种适合作为研究门脉高压症的动物模型。
To establish a clear and sustained portal pressure, collateral circulation and typical and reliable liver cell damage in animal models of portal hypertension. Methods Twelve healthy dogs were randomly divided into experimental group (n = 6) and control group (n = 6). In the experimental group, the main vein of the portal vein was narrowed 1/2 plus thread chronic embolization and intraperitoneal injection of carbon tetrachloride was performed one week after the operation. The sham operation and the same CCL4 intraperitoneal injection were performed in the experimental group. The levels of ALT, AST, TB and PT were measured. The mean number of lower esophageal blood vessels in each group before operation and on the 20th day after operation , Mean vascular diameter, portal pressure changes and liver histopathological changes. Results All dogs in the experimental group appeared abdominal varicose veins 15 days after operation, the incidence rate was 100%. The portal vein pressure, the average number of esophageal vessels and the mean vessel diameter of the experimental group at 20 days after operation increased compared with the control group (P <0.05) The liver injury scores of the group were significantly lower than those before operation (P <0.05), but there was no significant difference between the two groups. Conclusion This model of portal hypertension is obvious, hepatocellular injury is clear and typical, the end of the experiment clear, high success rate, is a suitable animal model for the study of portal hypertension.