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海龙、海马均为《中华人民共和国药典》收载品种,但均无理化质量标准。本文用7530—紫外分光光度计(上海分析仪器厂)测定了尖海龙、大海马、刺海马、三斑海马的紫外吸收光谱,四者图谱近似,说明各品种所含成分基本相同,其细微差异也可以作为鉴别各品种的依据。利用比色法测定了各药材胆甾醇含量,作为衡量海龙、海马质量的标准之一,本法在显色剂中加入硫脲,溶液颜色稳定,持续时间长,灵敏准确,专属性强。
Hailong and the seahorses are all varieties that are included in the Pharmacopoeia of the People’s Republic of China, but all have no physical and chemical quality standards. In this paper, the ultraviolet absorption spectrum of the sharp sea dragon, the sea horse, the thorn hippocampus and the three-spot hippocampus was measured with a 7530-ultraviolet spectrophotometer (Shanghai Analytical Instrument Factory). The four spectra were similar, indicating that the ingredients contained in the varieties were basically the same, with minor differences. Can also be used as the basis for identifying each variety. The colorimetric method was used to determine the content of cholesterol in each medicinal material and was used as one of the standards for measuring the quality of sea dragons and hippocampus. This method added thiourea to the color developing agent. The color of the solution was stable, the duration was long, the sensitivity was accurate, and the specificity was strong.