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本工作利用大鼠脊髓蛛网膜下腔注射的方法,观察了在脊髓水平5-HT 和吗啡的镇痛作用及其相互关系。结果如下:(1)脊髓蛛网膜下腔注射 200μg 5-HT 或10μg吗啡可产生明显的镇痛作用,并分别被 5-HT受体阻断剂肉桂硫胺和阿片受体阻断剂纳洛酮所对抗。(2)单纯使用肉桂硫胺可产生痛敏。纳洛酮对痛阈无明显影响。(3)5-HT 镇痛作用不能被大剂量纳洛酮(10mg/kg,sc)阻断。吗啡镇痛作用则可被脊髓蛛网膜下腔注射肉桂硫胺所削弱。后一效应可能与肉桂硫胺本身可引起痛敏有关。上述结果表明,在脊髓水平5-HT 和吗啡可通过各自的受体产生镇痛作用,两者间无明显依赖关系。5-HT 可能有紧张性活动,内源性阿片肽似不存在紧张性作用。
In this work, the spinal cord subarachnoid injection in rats was used to observe the analgesic effect of 5-HT and morphine on the level of the spinal cord and their correlation. The results are as follows: (1) Spinal subarachnoid injection of 200μg 5-HT or 10μg morphine can produce significant analgesic effect, and were blocked by the 5-HT receptor cinnamamine and opioid receptor blocker naloxone Ketone confrontation. (2) Simply use cinnamic thiamine can produce pain sensitivity. Naloxone had no significant effect on pain threshold. (3) 5-HT analgesic effect can not be blocked by high-dose naloxone (10mg / kg, sc). Morphine analgesic effect can be impaired by injection of cinnamaldehyde into the spinal subarachnoid space. The latter effect may be related to cinnamic thiamine itself can cause pain sensitivity. The above results indicate that 5-HT and morphine at the spinal level produce analgesic effects through their respective receptors with no obvious dependence on them. 5-HT may have nervous activity, endogenous opioid seems to be no tension effect.