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目的对成都汉族D3S1358,TH01,D21S11,D18551,PentaE,D55818,D13S317,D7S820,D16SS39,CSF1PO,Penta D,VWA,D8S1179,TPOX,FGA等15个STR基因座的遗传多态性进行群体遗传学研究。方法利用荧光标记复合扩增及毛细管电泳自动荧光检测的方法,对232名无关个体获得15个STR基因座等位基因的分布频率等群体遗传学数据。结果 D3S1358,TH01,D21S11,D18551,PentaE,D55818,D13S317,D7S820,D16SS39,CSF1PO,Penta D,VWA,D8S1179,TPOX,FGA等15个基因座上分别检出等位基因经检验在D3S1358、TH01、D21S11、D18551、PentaE、D55818、D13S317、D7S820、D16SS39、CSF1PO、Penta D、VWA、D8S1179、TPOX、FGA基因座上分别有8,6,16,16,20,9,8,8,8,8,11,12,8,6,18个等位基因。他们的等位基因频率分别在0.002-0.356、0.037-0.498、0.002-0.256、0.002-0.19、0.002-0.168、0.002-0.349、0.002-0.284、0.002-0.371、0.002-0.267、0.002-0.366、0.002-0.328、0.002-0.31、0.024-0.211、0.002-0.519、0.002-0.209之间。15个STR基因座的基因型在调查的群体中的分布符合Hardy-weinberg平衡,累积个人识别率(TDP)大于0.99999999,累积非父排除率(CEP)等于0.999999。结论这15个STR基因座多态性好,灵敏度高,可用于人类遗传分析及法医学中的亲子鉴定和个人识别。
Objective To study the genetic polymorphism of 15 STR loci including D3S1358, TH01, D21S11, D18551, PentaE, D55818, D13S317, D7S820, D16SS39, CSF1PO, PentaD, VWA, D8S1179, TPOX and FGA in Chengdu Han population . Methods Population genetic data of 15 STR loci alleles were obtained from 232 unrelated individuals using fluorescence-labeled multiplex amplification and capillary electrophoresis auto-fluorescence detection. Results The alleles were detected in D3S1358, TH01, D21S11, D18551, PentaE, D55818, D13S317, D7S820, D16SS39, CSF1PO, PentaD, VWA, D8S1179, TPOX, D21S11, D18551, PentaE, D55818, D13S317, D7S820, D16SS39, CSF1PO, PentaD, VWA, D8S1179, TPOX, FGA loci were respectively 8,6,16,16,20,9,8,8,8,8 , 11, 12, 8, 6, 18 alleles. Their allele frequencies were 0.002-0.356, 0.037-0.498, 0.002-0.256, 0.002-0.19, 0.002-0.168, 0.002-0.349, 0.002-0.284, 0.002-0.371, 0.002-0.267, 0.002-0.366, 0.328, 0.002-0.31, 0.024-0.211, 0.002-0.519, 0.002-0.209. The genotypes of the 15 STR loci in the surveyed population were in Hardy-weinberg equilibrium with a cumulative personal recognition rate (TDP) of greater than 0.99999999 and a cumulative non-father exclusion ratio (CEP) equal to 0.999999. Conclusion These 15 STR loci have good polymorphism and high sensitivity and can be used for paternity testing and personal identification in human genetic analysis and forensic science.