论文部分内容阅读
目的探讨双石汤对高血压大鼠心肌肥厚的作用及其机制。方法采用左肾动脉结扎法建立肾性高血压大鼠模型。实验分组:模型组、西药组、中药组、假手术组。中药组与西药组分别予双石汤及贝那普利灌胃,假手术组及模型组予等体积生理盐水。分别于术前、术后2周及用药4周、8周检测血压,用药8周后进行心脏超声检查,测定左室舒张末期内径(LVDd)及收缩末期内径(LVDs),左室舒张末期左室后壁厚度(LVPWd),左室收缩末期左室后壁厚度(LVPWs),射血分数(EF)。称取体重、全心重及左室重量,计算心重指数(CI)和左心室质量指数(LVMI),ELISA法检测血清中AngⅡ含量,PCR检测AT1R m RNA表达水平。结果与假手术组比较,中药组血压及LVDd、LVDs、LVPWd、IVSd降低(P<0.05),而EF升高(P<0.05);CI及LVMI降低(P<0.05);血清AngⅡ含量及AT1R的m RNA表达均下降(P<0.01)。结论双石汤能有效降低肾性高血压大鼠模型的血压,并通过抑制AngⅡ及其受体这一机制保护心肌。
Objective To investigate the effect and mechanism of Shuan Shi Tang on hypertrophy in rats with cardiac hypertrophy. Methods Left renal artery ligation was used to establish a renal hypertensive rat model. Experimental group: model group, western medicine group, traditional Chinese medicine group, sham group. The traditional Chinese medicine group and the western medicine group were given Shuangshi Decoction and benazepril orally, and the sham operation group and model group were given the same volume of normal saline. Blood pressure was measured at preoperative and postoperative 2 weeks, 4 weeks and 8 weeks after treatment, and echocardiography was performed 8 weeks after treatment. Left ventricular end diastolic diameter (LVDd), end systolic diameter (LVDs), left ventricular end diastolic dimension Posterior wall thickness (LVPWd), left ventricular posterior wall thickness (LVPWs) and ejection fraction (EF). Weigh body weight, total heart weight and left ventricular weight, calculate the heart weight index (CI) and left ventricular mass index (LVMI), serum Ang Ⅱ levels by ELISA, AT1R m RNA expression levels detected by PCR. Results Compared with the sham operation group, the blood pressure and LVDd, LVDs, LVPWd and IVSd decreased (P <0.05), EF increased (P <0.05), CI and LVMI decreased (P < M RNA expression decreased (P <0.01). Conclusion Shuangshi decoction can effectively reduce the blood pressure in renal hypertensive rat model and protect the myocardium by inhibiting Ang Ⅱ and its receptor.