论文部分内容阅读
本研究利用已知基因型的番茄材料,筛选出抗番茄黄化曲叶病Ty-3基因紧密连锁的分子标记SCAR1、抗枯萎病I-2基因紧密连锁的分子标记SCAR2和抗根结线虫病Mi基因紧密连锁的分子标记SCAR3,并应用这3个分子标记建立能同时鉴定Ty-3、I-2和Mi基因的多重PCR体系。经多次验证,扩增的特异性片段与单引物扩增片段一致,其结果准确可靠,可用于同时对3个抗病基因的鉴定。利用该体系对300份番茄材料进行种质资源筛选,结果表明分子检测结果与接种鉴定结果几乎吻合。本研究建立的多重PCR方法简易、高效、快速,为番茄抗病材料的筛选、分子标记辅助聚合育种工作奠定了基础。
In this study, SCAR1, a molecular marker closely linked to the Ty-3 gene of Tolerance, was screened from tomato materials with known genotypes. SCAR1, a molecular marker closely linked to the Fusarium wilt disease I-2 gene, and root knot nematode resistance Mi gene closely linked to the molecular marker SCAR3, and using these three molecular markers to establish a multiplex PCR system that can simultaneously identify Ty-3, I-2 and Mi genes. After multiple verification, the amplified specific fragment is consistent with the single primer amplified fragment, the result is accurate and reliable, and can be used for the simultaneous identification of three disease-resistant genes. The system was used to screen 300 tomato materials for germplasm resources. The results showed that the molecular detection results almost coincided with the inoculation results. The multiplex PCR method established in this study was simple, efficient and rapid, which laid the foundation for the screening of tomato disease-resistant materials and molecular marker-assisted polymerization breeding.