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目的观察Bcl-2蛋白在外伤性癫痫病灶中的表达变化,探讨其作用机制。方法收集外伤性癫痫、非外性癫痫患者及交通事故死亡者脑皮质各15例,采用免疫组织化学和荧光免疫组织化学技术,观察各实验组Bcl-2蛋白表达水平,测定阳性表达的灰度值,数据采用SPSS 12.0软件进行统计分析。结果外伤性及非外伤性癫痫组Bcl-2蛋白表达水平均高于交通事故死亡组(P<0.05);而非外伤性癫痫组表达明显高于外伤性癫痫组,差异亦具有显著统计学意义(P<0.05)。结论 Bcl-2蛋白能抑制神经元凋亡,对外伤性癫痫的发病过程具有抑制作用。
Objective To observe the expression changes of Bcl-2 protein in traumatic epilepsy and to explore its mechanism. Methods 15 cases of traumatic epilepsy, non-epilepsy and traffic accident were collected respectively. The expression of Bcl-2 protein in each experimental group was observed by immunohistochemistry and fluorescence immunohistochemistry. The gray level of positive expression Value, the data using SPSS 12.0 software for statistical analysis. Results The expression of Bcl-2 protein in traumatic and non-traumatic epilepsy group was significantly higher than that in traffic accident death group (P <0.05), while the expression in non-traumatic epilepsy group was significantly higher than that in traumatic epilepsy group, the difference was also statistically significant (P <0.05). Conclusion Bcl-2 protein can inhibit neuronal apoptosis and has an inhibitory effect on the pathogenesis of traumatic epilepsy.