论文部分内容阅读
作者已制备了当归的抗主要补体活化果胶(AR-2IIb)的多克隆抗体(抗-AR-2IIb-IgG),该抗体含有AR-2IIb的“分支”区中的中性糖的侧链区。此次对该侧链区的糖链抗原决定基进行解析。 方法:①抗-AR-2IIb-IgG:按已报道的方法制备、精制抗体。②“分支”区:通过用内-α-(1→4)-聚半乳糖醛酸酶消化AR-2IIb予以制备。
The authors have prepared Angelica’s polyclonal antibody (anti-AR-2IIb-IgG) against primary complement-activated pectin (AR-2IIb) that contains the side chains of neutral sugars in the “branch” region of AR-2IIb. Area. This time, the sugar chain epitopes in this side chain region were analyzed. Methods: 1 Anti-AR-2IIb-IgG: The antibody was prepared and purified according to the reported method. 2 “branch” region: Prepared by digestion of AR-2IIb with endo-α-(1→4)-galacturonase.