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目的 了解中国汉族人和法国人Ⅰ型胶原α链Spl结合序列MscⅠ酶切位点基因多态性分布的异同。方法 对 96例中国汉族人和 78例法国人用DXA骨密度仪进行BMD测量 ,同时采集全血并抗凝保存。用聚合酶链式反应、限制性酶切技术对Ⅰ型胶原α链Spl结合序列基因型进行分析。结果 96例汉族人的基因型均为SS ,78例法国人的基因型为 5 3例SS、2 2例Ss和 3例ss,经Fisherχ2 检验 ,显示差异有非常显著性 (P <0 0 1)。结论 汉族人和法国人的Ⅰ型胶原α链Spl结合序列MscⅠ酶切位点基因多态性显著不同。汉族人Ⅰ型胶原α链Spl结合序列MscⅠ酶切位点稀少。
Objective To understand the similarities and differences of the Msc Ⅰ gene polymorphism distribution between the Sp1-binding sequence and α-chain of Chinese Han and French. Methods BMD measurements were performed on 96 Chinese Han and 78 French on DXA bone densitometry, while whole blood was collected and stored anticoagulant. Polymerase chain reaction and restriction enzyme digestion were used to analyze the Spl binding genotypes of type I collagen a chain. Results The genotypes of 96 Han Chinese were all SS. The genotype of 78 Han French was 53 cases of SS, 22 cases of Ss and 3 cases of SS. The Fisherχ2 test showed that there was significant difference (P <0.01 ). Conclusion Han and French type Ⅰ collagen α chain Spl binding sequence Msc Ⅰ restriction site gene polymorphism significantly different. Han type Ⅰ collagen α chain Spl binding sequence Msc Ⅰ restriction sites rare.