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目的为研究角蛋白克隆抗体免疫组织化学法在检测淋巴结微转移灶上的意义。方法采用三种角蛋白单克隆抗体AE1,AE3、AE1/AE3,对我院47例大肠癌术后患者331个淋巴结用免疫组织化学法重新检查。结果36个淋巴结存在微转移灶(11.9%),Duke’sC期患者微转移灶检出率高于Duke’B期患者(P<0.05)。随访显示有微转移灶Duke’B期病人肿瘤复发、远处扩散转移均较无微转移病人高(P<0.05)。结论即使是组织学检查结果为阴性的淋巴结,也很有存在微转移的可能性。免疫组织化学法栓测单克隆抗角蛋白抗体,可详细阐明临床分期,并可提供大肠癌治疗的指征。
Objective To investigate the significance of immunohistochemical staining of keratin antibody in the detection of lymph node micrometastasis. Methods Three keratin monoclonal antibodies AE1, AE3, AE1/AE3 were used to reexamine 331 lymph nodes in 47 patients with colorectal cancer in our hospital by immunohistochemical method. Results There were micrometastases in 36 lymph nodes (11.9%), and the detection rate of micrometastases was higher in Duke’sC patients than in Duke’B patients (P<0.05). Follow-up showed that patients with micrometastasis in Duke’B stage had higher tumor recurrence and distant metastasis than those without micrometastasis (P<0.05). Conclusion Even lymph nodes with negative histological findings are likely to have micrometastatic potential. Immunohistochemical detection of monoclonal anti-keratin antibodies can elucidate the clinical stage and provide indications for the treatment of colorectal cancer.