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目的:明确骨髓间充质干细胞(MSCs)对内毒素血症小鼠的治疗作用。方法:动物分为4组:对照组、内毒素血症组、间充质干细胞治疗组、间充质干细胞组。分别在内毒素脂多糖(LPS)注射24 h和7 d后观察小鼠心功能的变化,ELISA方法检测血清细胞因子的水平,组织学方法观察对心肌、肝脏、肺脏和肾脏形态学改变的影响。结果:与对照组小鼠相比,内毒素血症组经LPS刺激后血清白介素1β(IL-1β)和肿瘤坏死因子α(TNF-α)含量增高,间充质干细胞治疗组血清IL-1β和TNF-α含量明显降低;内毒素血症组小鼠心功能明显下降,间充质干细胞治疗组心功能明显恢复;内毒素血症小鼠心肌细胞和肝细胞凋亡增加,肺间质和肺泡水肿,间充质干细胞治疗组上述组织损伤明显改善。结论:MSCs移植抑制了内毒素血症小鼠的炎症反应,改善了心功能,减轻了对心脏、肝脏和肺脏的损害。
Objective: To clarify the therapeutic effect of bone marrow mesenchymal stem cells (MSCs) on endotoxemia mice. Methods: Animals were divided into 4 groups: control group, endotoxemia group, mesenchymal stem cell treatment group and mesenchymal stem cell group. The changes of cardiac function in mice were observed after LPS injection for 24 h and 7 d respectively. Serum levels of cytokines were detected by ELISA. The morphological changes of myocardium, liver, lung and kidney were observed by histological method . Results: Compared with the control group, the levels of serum IL-1β and TNF-α in LPS group were significantly increased after LPS stimulation. The levels of IL-1β The levels of TNF-α and TNF-α in the endotoxemia group were significantly lower than those in the endotoxemia group. The cardiac function of the mice in the endotoxemia group was significantly decreased. The cardiac function of the mice in the mesenchymal stem cell group was significantly restored. The apoptosis of cardiomyocytes and hepatocytes in endotoxemia mice was increased, Alveolar edema, mesenchymal stem cell treatment group of tissue damage was significantly improved. CONCLUSIONS: MSCs transplantation inhibits inflammatory response in endotoxemic mice, improves cardiac function, and reduces damage to the heart, liver and lung.