一、前言单克隆抗体技术是近十年来迅速发展起来的一项重大生物学技术,也是免疫学中的一项革命。在体内,由一个B 细胞衍生的所有浆细胞属于一个纯系,或称“克隆”。用同一克隆的所有细胞产生的抗体都是对同一抗原决定簇特异的,具有相同的化学结构和生物活性。1975年,Kuhler 和Milstein 将免疫B 细胞和能够在体外长期培养的肿瘤细胞,在融合剂的作用下,融合成了兼有两种亲本特性的细胞。这种杂交瘤细胞既能产生对免疫原特异的抗体, I. Introduction Monoclonal antibody technology is a major biological technique developed rapidly in recent ten years and is also a revolution in immunology. In the body, all plasma cells derived from one B cell belong to one pure line, or “clone.” Antibodies produced by all cells of the same clone are specific for the same epitope and have the same chemical structure and biological activity. In 1975, Kuhler and Milstein fused immune B cells and tumor cells that can be cultured in vitro long after fusing to form cells that combine both parental properties. This hybridoma cells produce both antibodies that are specific for the immunogen,