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从海南文昌采集23种红树植物的根际土壤,采用GA、HV作为选择性分离培养基,添加复合维生素、放线菌酮、制霉菌素和重铬酸钾,结合适当的预处理:干热120°C60min或干热100°C60min及1%氯胺-T处理30min,平板稀释涂布法(10-1、10-2、10-3)分离其中的小双孢菌。共分离得到199株放线菌,通过培养特征及显微形态观察,发现其中有链霉菌147株、非链霉菌52株。选择7株链霉菌和28株非链霉菌进行了16SrRNA基因序列分析,结果显示19株菌属于小双孢菌属,7株属于链霉菌属,4株属于野野村菌属,2株属于小单孢菌属,1株属于链孢囊菌属,1株属于阿萨诺氏菌属,1株属于小单孢菌科。结果表明红树林根际土壤中蕴含着丰富的小双孢菌资源。
Rhizosphere soils of 23 species of mangrove plants were collected from Wenchang, Hainan Province. GA, HV were used as the selective medium for isolation, and multivitamins, cycloheximide, nystatin and potassium dichromate were added, and the appropriate pretreatment Heat 120 ° C60min or dry heat 100 ° C60min and 1% chloramine-T treatment 30min, plate dilution coating method (10-1, 10-2, 10-3) to isolate one of them. A total of 199 strains of actinomycetes were isolated. According to the characteristics of culture and microscopic observation, 147 strains of Streptomyces and 52 strains of non-Streptomyces were found. Seven strains of Streptomyces and 28 strains of non-Streptomyces were selected for sequence analysis of 16S rRNA gene. The results showed that 19 strains belonged to Micrococcus, 7 strains belonged to Streptomyces, 4 belonged to Yemen and 2 were small 1 strain belonging to the genus Neurospora, 1 belonging to Aspergillus and 1 belonging to the genus Micromonospora. The results showed that mangrove rhizosphere soil is rich in Microciscycles resources.