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目的:建立同时测定五味子种子挥发油中α-蒎烯、β-蒎烯、月桂烯、柠檬烯、乙酸龙脑酯和榄香烯6种成分含量的气相色谱法。方法:采用水蒸气蒸馏法进行挥发油的提取,采用GC对挥发油中α-蒎烯、β-蒎烯、月桂烯、柠檬烯、乙酸龙脑酯和榄香烯6种成分进行含量测定。气相色谱条件:采用HP-1701石英毛细管色谱柱(30m×320μm×1μm);氢火焰离子化检测器(FID);进样口温度230℃;检测器温度250℃;柱温以60℃为起始温度,以10℃.min-1升温至125℃,保持5min,再以10℃.min-1升至150℃,保持2min;载气为氮气,流速为0.9mL.min-1;进样方式为分流进样,分流比10∶1;进样量为1μL。结果:α-蒎烯、β-蒎烯、月桂烯、柠檬烯、乙酸龙脑酯和榄香烯进样浓度分别在0.0386～0.9640g.L-1(r=0.9999),0.0458～0.6870g.L-1(r=0.9996),0.0411～1.0270g.L-1(r=0.9998),0.450～0.9004g.L-1(r=0.9998),0.4043～4.0425g.L-1(r=0.9996),0.0252～0.6300g.L-1(r=0.9999)范围内呈良好线性关系;平均回收率(n=6)分别为α-蒎烯99.48%(1.21%)、β-蒎烯99.10%(1.22%)、月桂烯100.28%(1.49%)、柠檬烯100.02%(1.57%)、乙酸龙脑酯101.68%(1.96%)和榄香烯100.75%(1.61%)。结论:方法简便、快速、准确,可作为五味子种子挥发油质量控制的指标。
OBJECTIVE: To establish a gas chromatographic method for the simultaneous determination of 6 constituents of α-pinene, β-pinene, myrcene, limonene, bornyl acetate and elemene in the essential oil of Schisandra chinensis seeds. Methods: The volatile oil was extracted by steam distillation. The contents of α-pinene, β-pinene, myrcene, limonene, bornyl acetate and elemene in volatile oil were determined by GC. Gas chromatographic conditions: HP-1701 quartz capillary column (30m × 320μm × 1μm); flame ionization detector (FID); inlet temperature 230 ℃; detector temperature 250 ℃; The temperature was raised to 125 ° C. at 10 ° C.min-1 for 5 min and then to 150 ° C. at 10 ° C.min-1 for 2 min. The carrier gas was nitrogen and the flow rate was 0.9 mL · min -1. The injection The way for the split injection, split ratio of 10: 1; injection volume of 1μL. Results: The concentrations of α-pinene, β-pinene, myrcene, limonene, bornyl acetate and elemene were 0.0386-0.9640gL-1 (r = 0.9999) and 0.0458-0.66870gL-1 (R = 0.9998), 0.4043 to 4.0425 gL-1 (r = 0.9996), 0.0252 to 0.6300 gL-1 (r = 0.9998) (N = 6) were 99.48% (1.21%) of α-pinene, 99.10% (1.22%) of β-pinene, 100.28% (1.49%) of myrcene, 100.02% (1.57%), bornone acetate 101.68% (1.96%) and elemene 100.75% (1.61%). Conclusion: The method is simple, rapid and accurate and can be used as an indicator of the quality control of volatile oil from Schisandra chinensis seeds.