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目的:观察三肽化合物酪丝亮肽的抗肿瘤作用,探讨其对单核巨噬细胞的激活作用。方法:观察YSL对人肝癌BEL-7402裸鼠移植瘤的抑制作用;观察YSL对体外培养。BEL-7402细胞体系的抑制作用;观察YSL对PEMφ杀伤肿瘤细胞BEL-7402及B16-F10的影响;观察YSL对PEMφ分泌合成IL-1β,FNF-α和NO等细胞毒效应分子的影响。结果:YSL能显著抑制BEL-7402移植瘤裸鼠的肿瘤生长,给药剂量为160μg/(kg·d)时疗效最显著,抑制率为44.03%;YSL体外对BEL-7402细胞生长有一定的抑制作用,与阴性对照组比较有显著性差异(P<0.05);YSL能增强裸鼠PEMφ对BEL-7402,B16-F10杀伤作用,与生理盐水对照组相比有显著性差异(P<0.05);YSL能增强Balb/c小鼠PEMφ对BEL-7402,B16-F10杀伤作用,与生理盐水对照组相比有显著性差异(P<0.05);YSL能促进小鼠PEMφ分泌合成细胞毒效应分子IL-1β,TNF-α和NO,与生理盐水对照组相比有显著性差异(P<0.05)。结论:YSl能够抑制BEL-7402的增殖,增强单核巨噬细胞的细胞毒功能,促进细胞霉效应分子IL-1β,TNF-α和NO的分泌合成。
Objective: To observe the antitumor effect of tripeptide tyroserleutide and investigate its activation on monocyte-macrophage. Methods: To observe the inhibitory effect of YSL on human hepatocellular carcinoma BEL-7402 xenografts in nude mice. The in vitro culture of YSL was observed. BEL-7402 cell system. The effect of YSL on the killing of tumor cells BEL-7402 and B16-F10 by PEMφ was observed. The effect of YSL on the secretion of IL-1β, FNF-α and NO by PEMφ was observed. Results: YSL could significantly inhibit the tumor growth of BEL-7402 xenografts in nude mice, and the most effective dose was 160μg / (kg · d) YSL with the inhibition rate of 44.03%. YSL had a certain effect on the growth of BEL-7402 cells in vitro (P <0.05). YSL enhanced the killing effect of PEMφ on BEL-7402 and B16-F10 in nude mice, which was significantly different from that of saline control group (P <0.05) ); YSL enhanced the killing effect of PEMφ on BEL-7402 and B16-F10 in Balb / c mice compared with that of saline control group (P <0.05); YSL promoted the secretion of PEMφ There were significant differences in IL-1β, TNF-α and NO between the two groups (P <0.05). CONCLUSION: YSl can inhibit the proliferation of BEL-7402, enhance the cytotoxicity of monocyte-macrophage cells and promote the secretion and synthesis of the cytokines IL-1β, TNF-α and NO.