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目的:检测人脊椎骨中是否存在褪黑素受体mRNA的表达,并探讨其意义。方法:取手术切除的人脊椎松质骨,抽提总RNA。用逆转录酶-聚合酶链反应(RT-PCR)方法检测褪黑素受体MT1、MT2mRNA的表达。结果:人脊椎骨总RNA以MT1和MT2两种受体引物进行的RT-PCR产物电泳呈阳性条带,其基因片段长度与MT1和MT2受体引物之间的核酸长度相当。结论:人脊椎骨中存在MT1、MT2两种褪黑素受体mRNA的表达,证明脊椎骨是褪黑素作用的靶器官褪黑素可能通过存在于脊椎骨中的受体来调节脊柱的生长发育
Objective: To detect the existence of melatonin receptor mRNA in human vertebrae and explore its significance. Methods: The spine cancellous bone was excised and the total RNA was extracted. The expression of melatonin receptors MT1 and MT2 mRNA was detected by reverse transcriptase-polymerase chain reaction (RT-PCR). Results: RT-PCR products of human vertebra total RNA were positive by two kinds of MT1 and MT2 primer, and the length of gene fragment was the same as that between MT1 and MT2 receptor primer. CONCLUSION: The expression of MT1 and MT2 mRNAs in human vertebrae is evidenced that spinal vertebra is the target organ of melatonin. Melatonin may regulate the growth and development of the spine through the receptors present in vertebrae